期刊论文

Zhang, X.-Y.

中文

中国药理学通报

1001-1978

2006

22

1

101-105

10.3321/j.issn:1001-1978.2006.01.025

国家重点基础研究发展计划（973规划）资助项目（NoG2000056905）；

本校为其他机构

目的 比较非肽类血管紧张素Ⅱ受体Ⅰ型阻断剂氯沙坦（Losartan）和血管活性肽降钙素基因相关肽（CGRP）对血管紧张素Ⅱ诱导的血管平滑肌细胞增殖作用的影响，为探讨此两类物质的降压机制提供实验依据，方法 采用MTT，^3H-参入法和流式细胞仪分别测定血管紧张素Ⅱ刺激下，Losartan或CGRP干预下血管平滑肌细胞的增殖变化，Western bloting法测定不同状态下血管平滑肌细胞内ERK1／2的活性变化。结果 Losartan或CGRP能抑制血管紧张素Ⅱ刺激下血管平滑肌细胞的生存率、DNA合成、细胞周期增殖指数，以及细胞内ERKl／2的活性，并呈剂量依赖性。而且，CGRP抑制作用强于Losartan。结论 Losartan或CGRP能抑...

Aim: To compare the effects of the non-peptide angiotensin II receptor type I antagonist, Losartan, and the active vascular peptide, calcitonin gene-related peptide (CGRP), on the proliferation of vascular smooth muscle cells induced by angiotensin II, and to explore the mechanism of depressor effect of Losartan and CGRP in vivo. Methods: MTT, [3H]-Thymidine incorporation and flow cytometry, were used to determine the ability of proliferation of VSMC induced by angiotensin II in the presence or absence of Losartan or CGRP, Western blotting was used to determine the activity of ERK1/2. Results:...