期刊论文

Wang, C.

中文

中国药理学通报

1001-1978

2006

22

8

947-951

10.3321/j.issn:1001-1978.2006.08.013

国家自然科学基金资助课题（No 30470719）

本校为第一且通讯机构

目的 探讨巨噬细胞集落刺激因子(M-CSF)致动脉粥样硬化的作用是否与其影响基质金属蛋白酶(MMP)表达和活性改变有关及其可能机制.方法 应用明胶酶图分析方法观察M-CSF和(或)PD98059对体外培养的RAW 264.7细胞MMP-9活性的影响;Wester blot检测M-CSF和(或)PD98059对体外培养的RAW 264.7细胞p-ERK1/2表达的影响.结果 M-CSF能增强RAW 264.7细胞MMP-9的活性,并呈一定的剂量依赖性;同时M-CSF也呈时间、浓度依赖性促进p-ERK1/2的表达;PD98059不仅阻断了ERK1/2的磷酸化,而且也降低了MMP-9的活性.结论 M-CSF可诱导RAW 264.7细胞MMP-9的活性增加,其机制可能与M-CSF激活MAPK-ERK1/2通路有关.

Aim: To study the effect of Macrophage colony-stimulating factor(M-CSF) on MMP-9 in RAW 264.7 cell and explore the relationship between atherosclerosis caused by M-CSF and the activity of MMP-9. Methods: Gelatin zymography analysis was used to investigate the effect of M-CSF and PD98059 on the activity of MMP-9 in cultured RAW 264.7 cell. Western blot was used to study the effect of M-CSF and PD98059 on the express of p-ERK1/2 in cultured RAW 264.7 cell. Results: The enzyme activity of MMP-9 was significantly increased after 24-hour M-CSF treatment. Meanwhile, M-CSF upregulated the expression ...