期刊论文

Zhang, Y.

中文

中国药理学通报

1001-1978

2005

21

7

833-837

10.3321/j.issn:1001-1978.2005.07.017

湖南省自然科学基金资助项目（No02JJY2026）；

本校为第一且通讯机构

目的分析三氧化二砷(As2O3)对人胃癌MGC803细胞端粒重复序列结合因子1、2(TRF1、TRF2)表达及端粒稳定性的影响,探讨As2O3诱导细胞凋亡的机制.方法体外培养MGC803细胞,MTT比色实验分析As2O3对MGC803细胞增殖抑制作用,流式细胞术检测MGC803细胞凋亡,进行染色体端-端融合分析及Western blot检测TRF1、TRF2蛋白表达.结果 MTT法显示As2O3对人胃癌MGC803细胞有明显的抑制作用,且呈时间、剂量依赖关系.流式细胞术检测出现典型的凋亡峰,并随As2O3浓度增加和作用时间延长而增高,对照组未见此改变.染色体端-端融合分析显示5 μmol·L-1 As2O3处理MGC803细胞48 h染色体融和率明显高于对照组.Western blot分...

Aim: To study the effect of As2O3 (arsenic trioxide) on the expressions of telomeric repeat binding factor1,2 and telomeric stability of human gastric cancer MGC803 cells, and explore the mechanism of cell apoptosis. Method: MGC803 cell growth inhibition was measured with MTT assay. Apoptosis was analyzed with flow cytometry. Influence on chromosome distal end was analyzed with chromosome end - end fusion analysis. Expressions of TRF1 and TRF2 were determined with Western blot analysis. Results: MTT assay showed that As2O3 clearly inhibited the...