期刊论文

Tang, C.-K.

中文

中国药理学通报

1001-1978

2003

19

10

1177-1182

10.3321/j.issn:1001-1978.2003.10.025

湖南省科技厅重点资助项目 No 0 1SSY10 0 3；

本校为第一且通讯机构

目的　以THP 1巨噬细胞源性泡沫细胞为研究对象 ,探讨磷酸二酯酶抑制剂rolipram对THP 1巨噬细胞源性泡沫细胞ABCA1表达及细胞内胆固醇流出的影响。方法　用液体闪烁计数器检测细胞内胆固醇流出 ,运用逆转录 -多聚酶链反应和Westernblot印迹分别检测ABCA1mRNA与ABCA1蛋白的表达 ,采用低pH值EIA法测定细胞内cAMP水平。结果　实验显示PDE4抑制剂rolipram能引起THP 1巨噬细胞源性泡沫细胞cAMP水平、ABCA1表达和apoA 1介导的胆固醇流出成平衡增加 ,而细胞内总胆固醇、游离胆固醇与胆固醇酯明显减少。结论　PDE4抑制剂rolipram增加THP 1巨噬细胞源性泡沫细胞ABCA1表达及细胞内胆固醇流出 ,这为防治动脉...

AIM: To study the effect of rolipram on ATP binding cassette transporter 1 and cholesterol efflux in THP-1 macrophage-derived foam cell. METHODS: After exposure of the cultured THP-1 macrophage-derived foam cell to rolipram for different time, cholesterol efflux and ABCA1 mRNA and protein level were determined by FJ-2107P type liquid scintillator and reverse trancriptase-polymerase chaim reaction (RT-PCR) and western blot, respectively. RESULTS: Rolipram promoted cholesterol efflux in THP-1 macrophage-derived foam cell with time; RT-PCR and western blot showed that exposure of the cultured THP...