期刊论文

Dong, L.

中文

中国药理学通报

1001-1978

2002

18

1

43-45

10.3321/j.issn:1001-1978.2002.01.013

湖南省教委科研基金资助课题 ,No 96 15 5；

本校为第一且通讯机构

目的研究甲基莲心碱(Nef)对耐阿霉素人乳腺癌细胞MCF-7/Adr内阿霉素(ADM)积累的影响.方法 MTT法检测Nef对多药耐药性(MDR)的逆转作用,高效液相色谱(HPLC)法检测细胞内阿霉素浓度.结果 Nef能降低阿霉素对MCF-7/Adr细胞IC50值,能提高MCF-7/Adr细胞内ADM的浓度.结论 Nef逆转MCF-7/Adr细胞多药耐药的机制与其增加多药耐药细胞内抗癌药物积累有关.

AIM: To study the effect of neferine on intracellular adriamycin (ADM) accumulation in MCF-7 / Adr cell line. METHODS The cytotoxic effect was determined by MTT assay. The intracellular ADM concentration was assayed by HPLC. RESULTS: Neferine (Nef) decreased the IC50 of ADM to MCF-7 / Adr cells but increased the intracellular concentration of ADM. CONCLUSION: The mechanism of the MDR reversal effect of Nef is associated with the increase of ...