期刊论文

He, X.-S.

中文

中国全科医学

1007-9572

2017

20

17

2085-2088 and 2094

10.3969/j.issn.1007-9572.2017.17.009

81172210:国家自然科学基金 B2013-048:湖南省卫生计生委课题

本校为第一且通讯机构

目的 明确miR-142-3p能否通过靶向CD133的表达进而抑制鼻咽癌细胞增殖和侵袭能力,探讨miR-142-3p在抑制肿瘤生长中的分子机制.方法 于2015年7月-2016年9月,构建CD133 mRNA 3′-UTR荧光素酶报告载体,通过荧光素酶报告系统观察miR-142-3p对CD133 mRNA 3′-UTR荧光素酶活性的影响.将对照control、CD133 siRNA及miR-142-3p模拟物各40 μmol/L分别转染至鼻咽癌CNE2细胞,采用Western blotting法检测其对CD133表达水平的影响;设立阴性对照组〔瞬时转染对照control(40 μmol/L)〕、阳性对照组〔瞬时转染CD133 siRNA(40 μmol/L)〕、miR-142-3p组〔瞬时转染miR-142-3p模拟物(40 μmol/L)〕、联合组〔瞬时...

Objective: To investigate whether miR-142-3p can suppress the proliferation and invasion abilities of nasopharyngeal carcinoma cells by targeting CD133, in order to reveal the molecular mechanism that miR-142-3p functions as a tumor suppressor in nasopharyngeal carcinoma. Methods: This study was conducted between July 2015 and September 2016. CD133 mRNA 3'-UTR-luciferase vector was constructed and dual-luciferase reporter gene assay was employed to examine the effect of miR-142-3p on luciferase activity of CD133 mRNA 3'-UTR. Nasopharyngeal carc...