ERA-CRISPR/Cas12a-based, fast and specific diagnostic detection for Chlamydia pneumoniae

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Zhou, Yanxia;Yan, Zijun;Zhou, Shi;Li, Weiwei;Yang, Hongyu;...

通讯作者：

Wu, YM

作者机构：

[Yan, Zijun; Wu, Yimou; Zhou, Yanxia; Zhou, Shi; Zeng, Qilin; Yang, Hongyu; Wu, YM; Sun, Peiyuan] Univ South China, Inst Pathogen Biol, Hengyang Med Sch, Hengyang, Peoples R China.

[Li, Weiwei] Second Peoples Hosp Foshan, Dept Clin Lab, Foshan, Peoples R China.

[Chen, Hongliang] First Peoples Hosp Chenzhou, Dept Infect Control, Chenzhou, Peoples R China.

[Deng, Zhongliang] Univ South China, Coll Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang, Peoples R China.

通讯机构：

[Wu, YM ] U

Univ South China, Inst Pathogen Biol, Hengyang Med Sch, Hengyang, Peoples R China.

语种：

英文

关键词：

Chlamydia pneumoniae;OmpA gene;CRISPR/Cas12a;ERA;pathogenic bacteria detection;trans-cleavage

期刊：

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

ISSN：

2235-2988

年：

2024

卷：

页码：

1477422

DOI：

10.3389/fcimb.2024.1477422

基金类别：

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. The construct program of the key discipline in Hunan province for Preclinical Medicine and Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study (Hunan Provincial Education Department document, approval number: 2012-1 and 2014-405).

机构署名：

本校为第一且通讯机构

院系归属：

医学院

公共卫生学院

摘要：

Chlamydia pneumoniae (C. pneumoniae) is a specialized intracellular parasitic pathogen capable of causing pneumonia, sinusitis, bronchitis, and other respiratory diseases, which pose significant public health challenges. Therefore, rapid, accurate, and sensitive diagnosis is crucial for the prevention and treatment of respiratory diseases caused by C. pneumoniae. In this study, we combined enzymatic recombination amplification (ERA) with the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) 12a s...

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ERA-CRISPR/Cas12a-based, fast and specific diagnostic detection for Chlamydia pneumoniae

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