期刊论文

中文

解剖学报

0529-1356

2022

53

2

155-159

10.16098/j.issn.0529-1356.2022.02.003

湖南省自然科学基金省市联合项目(2018JJ4034) 湖南省自然科学基金青年基金(2018JJ3464) 湖南省教育厅优秀青年项目(19B576) 南华大学大学生研究性学习和创新性实验计划项目(X2019142)。

本校为第一机构

目的探讨海马脑源性神经营养因子前体(proBDNF)在社会隔离所致小鼠认知障碍中的作用。方法30只4周龄C57BL/6 J雄性小鼠随机分成正常群居组(GH,n= 15)和社会隔离组(SI,n = 15) 。各组于相同的环境饲养,GH组5只/笼,SI组1只/笼。采用新旧事物识别和新旧位置识别实验检测小鼠认知功能。Real-time PCR检测各组小鼠海马脑源性神经营养因子(BDNF) mRNA表达变化。Western blotting检测海马组织BDNF及proBDNF蛋白表达。基质金属蛋白酶9 (MMP-9)、组织型纤溶酶原激活剂(tPA)是胞外酶,可催化proBDNF转化为成熟BDNF。Real-time PCR检测BDNF转化酶MMP-9和tPA mRNA表达变化。结果行为学结果显示,与GH组相比,SI...

Objective To investigate the effect of hippocampal brain derived neurotrophic factor precursor(proBDNF) in cognitive dysfuction induced by social isolation. Methods Thirty C57 BL/6 J male mice(4-week old) were randomly divided into group house(GH,n=15) and socially isolated(SI,n=15) groups. The GH group(5 mice/cage) and SI group(1 mice/cage) were reared separately under the same conditions. The novel object recognition test and the novel place recognition test were used to evaluate the cognitive function. The expression of BDNF mRNA in the hippocampus was detected by Real-time PCR. The express...