期刊论文

中文

解剖学报

0529-1356

2022

53

2

155-159

10.16098/j.issn.0529-1356.2022.02.003

2018JJ4034:湖南省自然科学基金省市联合项目 2018JJ3464:湖南省自然科学基金 19B576:湖南省教育厅优秀青年项目 X2019142:南华大学大学生研究性学习和创新性实验计划

本校为第一且通讯机构

目的探讨海马脑源性神经营养因子前体(proBDNF)在社会隔离所致小鼠认知障碍中的作用。方法30只4周龄C57BL/6 J雄性小鼠随机分成正常群居组(GH,n= 15)和社会隔离组(SI,n = 15) 。各组于相同的环境饲养,GH组5只/笼,SI组1只/笼。采用新旧事物识别和新旧位置识别实验检测小鼠认知功能。Real-time PCR检测各组小鼠海马脑源性神经营养因子(BDNF) mRNA表达变化。Western blotting检测海马组织BDNF及proBDNF蛋白表达。基质金属蛋白酶9 (MMP-9)、组织型纤溶酶原激活剂(tPA)是胞外酶,可催化proBDNF转化为成熟BDNF。Real-time PCR检测BDNF转化酶MMP-9和tPA mRNA表达变化。结果行为学结果显示,与GH组相比,SI...

[Abstract] Objective To investigate the effect of hippocampal brain derived neurotrophic factor precursor (proBDNF) in cognitive dysfuction induced by social isolation. Methods Thirty C57BL / 6 J male mice (4-week old) were randomly divided into group house (GH,n = 15) and socially isolated (SI,n = 15) groups. The GH group (5 mice / cage) and SI group (1 mice / cage) were reared separately under the same conditions. The novel object recognition test and the novel place recognition test were used to evaluate the cognitive function. The expressio...