期刊论文

中文

解剖学报

0529-1356

2022

53

4

412-417

10.16098/j.issn.0529-1356.2022.04.002

湖南省自然科学基金(青年项目)(2018JJ3464)。

本校为第一机构

目的探讨溴结构域蛋白4(BRD4)抑制剂JQ1在甲醛诱导炎性痛模型小鼠脊髓中的表达变化及作用。方法32只ICR小鼠随机分为生理盐水组、甲醛注射5 min、30 min和60 min组,每组8只,Western blotting (n = 4/组)和Real-time PCR(n = 4/组)检测各组小鼠脊髓BRD4的蛋白和mRNA的表达水平; 66只小鼠随机分为甲醛注射组,溶媒(DMSO)加甲醛注射组,以及6.25、12.5、25和50 mg/kg JQ1注射加甲醛溶液组,每组11只,观察BRD4抑制剂JQ1对每组小鼠自发性疼痛的影响(n = 11/组);免疫组织化学(n = 3/组) 、Real-time PCR(n = 4/组)或Western blotting(n = 4/组)的方法检测25 mg/kg JQ1对模型小鼠脊髓即早基因c-fos及谷氨酸...

Objective To explore the expression and role of bromodomain-containing protein 4(BRD4)in spinal cord of mice which suffered inflammatory pain induced by formaldehyde solution.Methods Thirty-two ICR mice were randomly divided into normal saline group and formaldehyde injection 5 minutes,30 minutes and 60 minutes groups,with 8 mice in each group.The expression of BRD4 protein and mRNA in spinal cord of mice in each group were detected by Western blotting(n=4/group)and Real-time PCR(n=4/group);66 mice were randomly divided into formaldehyde injection group,vehicle(DMSO)plus formaldehyde injection...