期刊论文

Voluntary wheel running prevents the formaldehyde induced negative emotion through up-regulation of hippocampal neurogenesis in mice

中文

解剖学报

0529-1356

2021

52

05

686-691

10.16098/j.issn.0529-1356.2021.05.003

湖南省教育厅优秀青年项目（17B230）;湖南省教育厅科学研究项目（20C1614）； 南华大学大学生研究性学习和创新性实验计划项目（2018XJXZ156,X2019138,2017XJXZ032）；

本校为第一机构

目的探讨自主跑轮运动对甲醛疼痛模型小鼠负性情绪的影响。方法30只昆明雄鼠随机分成生理盐水对照组(NS) 、甲醛组(F) 、自主跑轮运动+甲醛组(R+F) 。右后爪足底皮下注射0.4%甲醛建立疼痛模型, R+F组在模型建立之前置于小鼠跑轮运动装置3周。以实验小鼠舔足累计时间评价自发痛行为;采用旷场实验(OFT)和高架十字迷宫实验(EPM)检测小鼠焦虑样行为;采用被迫游泳实验(FST)检测小鼠抑郁样行为;采用免疫组织化学方法检测海马未成熟神经元标记物双皮层蛋白(DCX)的表达情况。结果自发痛评分结果显示,与NS组相比,F组小鼠出现典型的双相痛反应,与F组相比,R+F组小鼠的第Ⅱ相时段舔咬足累计时间明显缩短(P

Objective To investigate the effect of voluntary wheel running on negative affective of mice induced by formaldehyde.Methods Thirty male Kunming mice were randomly divided into three groups,including normal saline control group (NS),formaldehyde model group (F),and voluntary wheel running with formaldehyde injection group (R+ F).The pain model was established by right hindpaw intraplantar formalin injection,the mice of R+F group experienced voluntary wheel running for three weeks before intraplantar formaldehyde injection.The spontaneous pain behavior was determined by the cumulative time of l...