期刊论文

Dexmedetomidine alleviates formaldehyde induced pain by inhibiting astrocytes activation in spinal cord

中文

解剖学报

0529-1356

2021

52

05

681-685

10.16098/j.issn.0529-1356.2021.05.002

湖南省教育厅优秀青年项目（17B230）； 湖南省自然科学基金省市联合项目（2018JJ4034）； 南华大学大学生研究性学习和创新性实验计划项目（2018XJXZ156,X2019138）；

本校为第一机构

目的探讨右美托咪定(DEX)在0.1%甲醛诱导的急性炎性痛中的作用。方法将30只ICR雌性小鼠随机分成生理盐水对照(NS)组、甲醛(F)组、右美托咪定+甲醛(DEX+F)组。向右后爪足底注射25 μl 0.4%甲醛建立小鼠急性炎性痛模型,在甲醛注射前1 h,DEX+F组小鼠腹腔注射DEX,NS组和F组小鼠腹腔注射等体积生理盐水。以小鼠舔咬足累计时间评价自发痛行为。采用免疫组织化学法和Western blotting检测脊髓星形胶质细胞标记物胶质纤维酸性蛋白(GFAP)的表达。结果自发痛评分结果显示,与NS组相比,F组小鼠出现典型的双相痛反应;与F组相比,DEX+F组小鼠的第Ⅰ时相痛(P

Objective To investigate the effect of dexmedetomidine (DEX) on formaldehyde-induced acute inflammatory pain.Methods Thirty female ICR mice were randomly divided into three groups,including normal saline control (NS) group,formaldehyde(F) group and dexmedetomidine + formaldehyde (DEX+F) group.0.1% formaldehyde solution was injected into subcutaneous tissue of mice right hind paw to establish acute inflammatory pain model.An hour before formalin injection,mice were intraperitoneal injected with DEX in DEX+F group,and mice were intraperitoneal injected with equal volume saline in NS group and F ...