期刊论文

MCC950 alleviating inflammatory pain induced by formaldehyde through down-regulation of immediate early genes in mouse spinal cord

中文

解剖学报

0529-1356

2021

52

06

870-874

10.16098/j.issn.0529-1356.2021.06.005

湖南省教育厅优秀青年项目（17B230）； 湖南省自然科学基金青年基金（2018JJ3464）； 南华大学大学生研究性学习和创新性实验计划项目（2018XJXZ156,X2019138,2017XJXZ032）；

本校为第一机构

目的探讨MCC950对甲醛模型小鼠疼痛的影响及可能机制。方法72只ICR雄性小鼠随机分为生理盐水对照组(NS组),甲醛模型组(F组) 、不同浓度梯度MCC950干预组(6.25 mg /kg MCC950+F、12.5 mg /kg MCC950+F、25 mg /kg MCC950+F、50 mg /kg MCC950+F) 。以小鼠累计舔咬足时间评价自发痛行为;采用Real-time PCR方法检测小鼠脊髓即早基因,包括c-Fos、Arc、早期生长反应因子-1(Egr-1) mRNA的表达变化;采用免疫组织化学法检测脊髓背角内c-Fos的表达。结果自发痛评分显示,与NS组相比,F组小鼠出现典型双相痛;与F组相比, 12.5 mg /kg、25 mg /kg及50 mg /kg MCC950预处理均可显著缓解甲醛所致小鼠疼痛。Real-...

Objective To investigate the effect of MCC950 on inflammatory pain induced by formaldehyde in mice. Methods Seventy-two male ICR mice were randomly divided into six groups,including saline control group (NS), formaldehyde model group(F),different doses of MCC950 intervention group (6.25 mg /kg MCC950 + F,12.5 mg /kg MCC950+F,25 mg /kg MCC950+F,50 mg /kg MCC950+F). The spontaneous pain was determined by the cumulative time of licking paw in mice. The expressions of immediate early genes (IEGs),such as c-Fos,Arc,early growth response protein 1(Egr-1),in the spinal cord were determined by Real-ti...