期刊论文

Fluoxetine improving the depression-like behavior induced by chronic restraint stress by up-regulation the expression of bromodomaincontaining protein 4 in hippocampus of mouse

中文

解剖学报

0529-1356

2019

50

1

18-23

10.16098/j.issn.0529-1356.2019.01.004

2018JJ3460:湖南省自然科学基金青年项目 2018JJ3464:湖南省自然科学基金青年项目 14A125:湖南省教育厅重点项目 16A187:湖南省教育厅重点项目 C2016042:湖南省卫生计生委科研课题 2015XQD27:南华大学博士科研启动基金 2015XQD28:南华大学博士科研启动基金 2017XJXZ035:大学生研究性学习和创新性实验计划 2017XJXZ036:大学生研究性学习和创新性实验计划 2017XJXZ032:大学生研究性学习和创新性实验计划

本校为第一机构

目的探讨氟西汀(FLX)对慢性束缚应激(CRS)所致小鼠抑郁样行为及海马内溴结构域蛋白4(BRD4)表达的影响。方法24只雄性昆明小鼠随机分为生理盐水对照(NS)组、抑郁模型(CRS)组、氟西汀干预(CRS+FLX)组。慢性束缚应激3周建立小鼠抑郁模型,应激的第8天至第21天CRS+FLX组于应激前30 min腹腔注射氟西汀(10 mg/kg),NS组及CRS组注射等体积生理盐水。采用糖水偏好实验、喷糖实验、强迫游泳实验、新旧事物识别实验和旷场实验检测各组小鼠行为变化;采用Western blotting及Real-time PCR法检测小鼠海马BRD4蛋白和mRNA的表达情况。结果与NS组相比,CRS组小鼠表现出明显的抑郁样行为,包括糖水偏好百分比显著降低...

Objective To investigate the effect of fluoxetine (FLX) on the expression of bromodomain-containing protein 4 (BRD4) in the hippocampus induced by chronic restraint stress (CRS),with the depression-like behaviour also being determined. Methods Twenty-four male Kunming mice were randomly divided into normal saline(NS) group,CRS group and fluoxetine(FLX) intervention(CRS+FLX) group. The mice of the CRS group were subjected to 3 weeks chronic restraint stress. The mice of CRS+FLX group were treated with fluoxetine by intraperitoneal injection 30 m...