期刊论文

Effect and mechanism of caloric restriction on early social isolation-induced cognitive impairment in mice

Wan, W.

中文

解剖学报

0529-1356

2019

50

4

418-422

10.16098/j.issn.0529-1356.2019.04.003

2018JJ3460:湖南省自然科学基金青年项目 2018JJ3429:湖南省自然科学基金青年项目 2018JJ4034:湖南省自然科学基金省市联合项目 16A187:湖南省教育厅重点项目 17B230:湖南省教育厅优秀青年项目

本校为第一机构

目的探讨热量限制(CR)对早期社会隔离(SI)诱导的认知功能障碍的改善作用及其可能机制。方法3周龄昆明雄小鼠36只随机分成正常群居组(GH,n= 12) 、早期社会隔离组(SI,n = 12) 、热量限制干预组(SI +CR,n= 12) 。在相同条件下饲养GH组(6只/笼)和SI组(1只/笼),而SI + CR组(1只/笼)每隔1 d给予食物。采用旷场实验检测小鼠的活动度,新旧事物识别实验检测小鼠认知功能;免疫组织化学方法检测小鼠海马区小胶质细胞标记物离子钙结合接头分子1(IBA-1)的表达; Western blotting方法检测海马组织小胶质细胞激活标记物CD68及炎症因子白细胞介素-1β(IL-1β)的表达。结果在旷场实验中,3组小鼠的活动度无差异。...

Objective: To investigate the effect and mechanism of caloric restriction (CR) on early social isolation (SI) induced cognitive dysfunction in mice. Methods: Thirty-six kunming mice (3-weeks old) were randomly divided into group house (GH, n= 12), early socially isolated (SI, n= 12), caloric restriction and socially isolated (SI+CR, n= 12). The GH group (6 mice/cage) and SI group (1 mice/cage) were reared separately under the same conditions, while the SI+CR group (1 mice/cage) were given food every other day. The open field experiment was used...