期刊论文

Gan, R.

中文

中南大学学报(医学版)

1672-7347

2017

42

6

605-610

10.11817/j.issn.1672-7347.2017.06.001

国家自然科学基金（81372579）； 湖南省教育厅科学研究项目（17C1402,14C0998）； 湖南省研究生创新项目（CX2015B386）~~；

本校为第一且通讯机构

目的:探讨miR-503是否能通过调控bcl-2的表达增强BEL-7402细胞对顺铂的敏感性。方法:采用实时荧光定量PCR检测肝癌细胞中miR-503和bcl-2 mRNA的表达水平;Western印迹观察肝癌细胞中Bcl-2蛋白的表达水平;脂质体转染法将miR-503模拟物瞬时转染至BEL-7402细胞;生物信息学软件预测miR-503潜在靶基因;双荧光素酶活性验证miR-503潜在的靶位点;MTT法检测细胞药物敏感性的改变。结果:相比于HL-7702细胞,miR-503在BEL-7402细胞中表达水平下调,Bcl-2蛋白的表达水平上调;miR-503能够与bcl-2靶向结合,下调bcl-2的表达;miR-503转染组的细胞活力较miRNA阴性对照组显著降低。结论:MiR-503可能通过靶向bcl-2增强...

OBJECTIVE: To investigate effects of miR-503 on cisplatin sensitivity in BEL-7402 cells by targeting of bcl-2. Methods: MiR-503 and bcl-2 mRNA expression levels in hepatocellular carcinoma cells were measured by real-time quantitative (qRT)-PCR; Bcl-protein level was detected by Western blot; miR-503 mimics were transiently transfected to the BEL-7402 cells by liposome transfection; potential target genes of miR-503 were predicted by Bioinformatics software; miR-503 potential targets were validated by dual luciferase activity; and the cell viability was measured by MTT assay. Results: MiR-503 ...