期刊论文

Xie, L.

中文

中南大学学报(医学版)

1672-7347

2014

39

3

276-281

10.11817/j.issn.1672-7347.2014.03.009

湖南省科技厅资助项目(2009FJ3117).This work was supported by China Hunan Provincial Science and Technology Department

本校为其他机构

目的:通过研究PFT-α对隐睾生精细胞中p53和bcl-2/bax表达的影响,探讨生精细胞凋亡的分子机制。方法:将雄性SD大鼠随机分为假手术组、隐睾组、隐睾+p53抑制剂(p-fifty three inhibitor-alpha,PFT-α)组、隐睾+ PFT-α溶媒(DMSO)组。后三组建立单侧隐睾模型,后两组分别于大鼠腹腔内注射PFT-α和PFT-α溶媒,每天1次,定时定量。 7 d后处死所有大鼠,取双侧睾丸称湿重。观察手术侧睾丸生精细胞组织形态,采用TUNEL法结合流式细胞术(flow cytometry,FCM)检测生精细胞凋亡程度,免疫组织化学和Western 印迹分别检测p53和bcl-2/bax的表达。结果:隐睾+PFT-α组与隐睾组和隐睾+PFT-α溶媒组比较,生精上皮排列...

OBJECTIVE: To determine the molecular mechanism of germ cell apoptosis via investigating the effect of PFT-alpha on the expression of p53 and bcl-2/bax during experimental cryptorchid cell apoptosis. METHODS: Male Sprague-Dawley rats were assigned into 4 groups: a sham-operated group, a cryptorchid group, a cryptorchid+p53 inhibitor (p53 inhibitor-alpha, PFT-alpha) group, and a cryptorchid+dissolvent of PFT-alpha [dimethyl sulphoxide (DMSO)] group. Unilateral cryptorchidism was surgically induced in the rats of the cryptorchid group, PFT-alpha group, and cryptorchid+dissolvent of PFT-alpha gro...