The present study was supported from National Natural Science Foundation of China (grant number 82003802), the Natural Science Foundation of Hunan Province (grant number 2019JJ50542), the Science and Technology program of Hunan Health Commission (grant number 20201978), the China Scholarship Council (grant no. 201808430085), the Scientific Research Project of Hunan Provincial Education Department(grant number 21B0438), the Hengyang City Science and Technology Planning Project (grant number 202150063473), the Scientific Research Project of Hunan Provincial Health Commission (grant number 202202044140), the Undergraduate Research based Learning and Innovative Experimental Program of Hunan Province (grant number S202112650003 and S202112650005).