期刊论文

Effect of immature dendritic cells （imDC） induced by sinomenine on the peripheral blood Th1/Th2 cytokines of allogeneic rat renal transplantation

中文

中国医师杂志

1008-1372

2017

19

7

992-994

10.3760/cma.j.issn.1008-1372.2017.07.008

国家自然科学基金（81273754）~~；

本校为第一机构

目的 观察青藤碱(SN)诱导的供体来源未成熟树突状细胞(imDC)对受体鼠静脉血Th1/Th2细胞因子的影响,探讨SN诱导的imDC诱导移植免疫耐受的机制.方法 分别选用Wistar和SD大鼠作为肾移植供体和受体,术前受体经静脉输注imDC,利用显微外科血管吻合法建立大鼠单肾移植模型,以ELISA法测定IL-2、IL-4、IL-10及干扰素(IFN)-γ等的水平.结果 (1)移植手术成功率89.5％,动脉吻合时间为(12.5±5.7) min,静脉吻合时间为(17.3±3.4) min.(2)SN-imDC 106组IL-2、IFN-γ、IL-41及L-10含量分别为(17.25±3.41) pg/ml,(239.80 ±9.06) pg/ml,(337.60±25.07) pg/ml及(1 432.00±106.39) pg/ml.同浓度中,SN-imDC组IL-...

Objective To investigate the effect of immature dendritic cell （imDC） derived from donor-derived bone marrow induced by alkaloid sinomenine （SN） on the Th1/Th2 eytokines in venous blood of receptors, and to probe into the mechanism which imDC induced by SN can lead transplantation immune tolerance. Methods Inbred strain Wistar and Sprague Dawley （SD） rats were selected as kidney trans-plant donor and recipient, respectively. Vessel sutures of the microsurgery technique were used to build the bilaterally renal transplantation model of rats...