期刊论文

Inhibition of tumor growth by intramuscular administration of the canstatin gene delivered by electroporation

中文

中国医师杂志

1008-1372

2014

16

9

1186-1190

10.3760/cma.j.issn.1008-1372.2014.09.009

本校为第一机构

目的 构建人canstatin克隆载体及重组真核表达载体,观察电脉冲介导骨骼肌内canstatin基因导入对小鼠Lewis肺癌皮下移植瘤生长的影响.方法 用一步法RT-PCR钓取胚肝canstatin cDNA,克隆入真核表达载体pEGFP-N1,构建含重组质粒pEGFP-N1/canstatin;将该重组质粒通过电脉冲转入荷瘤Lewis肺癌小鼠肌肉内,应用逆转录PCR、荧光显微镜测量荧光强度、肿瘤称重、肿瘤体积测定及免疫组化的方法检测canstatin基因在体内的表达和抗肿瘤作用.结果 获得684 bp人canstatin基因,测序证明canstatin cDNA编码序列与Genbank中报道的序列一致.电脉冲介导的质粒基因导入效率较单纯质粒注射高30倍以上;转canstatin基因后...

Objective To construct human canstatin gene eukaryotic expression vector and investigate the therapeutic effect of intramuscular canstatin gene delivered by electroporation on tumor growth.Methods Canstatin cDNA was amplified from total RNA extracted from fresh fetal liver by reversing transcription polymerase chain reaction （RT-PCR）.The canstatin cDNA fragment was in serted into pEGFP-N1 eukaryotic expression vector.The recombination plasmid was delivered to the quadriceps of the mice with Lewis lung carcinomas by electroporation intramuscul...