期刊论文

The effects of B. adolescentis and L. acidophilus on proportion of Thl/Th2 in acute and convalescent UC-mice

中文

中国医师杂志

1008-1372

2009

11

6

724-726

10.3760/cma.j.issn.1008-1372.2009.06.002

本校为其他机构

目的 分析急性期和恢复期实验性结肠炎小鼠外周血Th1/Th2亚群比例以及青春双歧杆菌和嗜酸乳酸杆菌对其影响.方法 40只BABL/c小鼠自由饮用7d DSS造成急性实验性结肠炎小鼠模型,随机分为4组:阴性对照组(NS)、阳性对照组(SASP)、青春双歧杆菌组(BF0624)、嗜酸乳杆菌组(LT0637),10只/组,以及正常对照组(N)10只(未造模).一部分在实验第8天处死采血;另一部分继续正常饮水10 d,在实验第18天处死采血;分离淋巴细胞,用流式细胞术三色法测定Th1/Th2亚群比例.结果 正常小鼠外周血Th1/Th2比例在0.84-0.96之间,而DSS诱导的急性结肠炎小鼠Th1/Th2比例增加,NS组达到2.21±0.83.在恢复期同样处于较高的水平;青春...

Objective To observe and analyze the effect of B. adolescentis and L. acidophilus on the proportion of Th cell Th1/Th2 in peripheral blood of UC-mice in acute stage and recovery stage. Method 40 BABL/c mice were induced by 3% DSS water for 7 days free drinking and then with distilled water for 10 days. They were randomly allocated in 4 groups： NS group, SASP group, BF0624 group and LT0637 group, also the fifth group-10 normal animals. The blood of mice were collected by removing their eyes at day 8 and day 18, and then the mononuclear cells we...