期刊论文

中文

中华器官移植杂志

0254-1785

2012

33

8

466-469

10.3760/cma.j.issn.0254-1785.2012.08.006

湖南省自然科学基金（09JJ3061）； 美国中华医学会CMB基金（06837）；

本校为第一机构

目的 观察缺血再灌注损伤(IRI)对大鼠肝组织内细胞周期调控相关基因转录和表达的影响,探讨生长阻滞和DNA损伤修复基因(Gadd45a)和早期生长反应基因1(Egr-1)参与DNA损伤后修复的可能机制.方法 按随机成组对照设计将大鼠平均分为正常对照组、缺血组、再灌注30min、1h、2h、3h及24 h组,于相应时间点获取大鼠肝组织样本,将利用芯片实验筛选出的关键基因Gadd45a和Egr-1作为目的基因,并采用RNA印迹法和实时荧光定量逆转录聚合酶链反应(RT1PCR)验证基因芯片筛查的结果,采用荧光免疫组织化学染色检测冰冻新鲜肝组织样本Egr-1的表达,采用免疫组织化学染色检测肝组织样本Gadd45a的表达.结果 RNA印迹检测结...

Objective To explore the possible mechanism that Gadd45a and Egr-1 together participate in the DNA damage repair, by analyzing the influence of hepatic ischemia-reperfusion injury （IRI） on the mRNA and protein expression of hepatic cell cycle-related genes. Method Methods Forty-two SD rats were divided into normal control group, ischemia 30 rain group and reperfusion 30 rain, 1 h, 2 h, 3 h and 24 h groups. The hepatic specimens of rats at the corresponding time points after success of modeling were obtained. Chip experiment was used to screen...