期刊论文

中文

中华疾病控制杂志

1674-3679

2016

20

2

193-197

10.16462/j.cnki.zhjbkz.2016.02.022

81273026:国家自然科学基金 B2011-044:湖南省卫生厅科学研究项目

本校为第一机构

目的预测has-miR-16靶基因并对靶基因的分子功能、生物学过程和信号转导通路富集性分析,全面认识miR-16涉及的生命过程和疾病。方法通过阅读文献结合生物信息学预测的方法得出靶基因集合,用Cytoscape 3.2. 1软件和DAVID(the database for annnotation visualization and integrated discovery)数据库分别对靶基因GO(gene ontology)注释和KEGG(kyoto encyclopedia of genes and genomes)信号转导通路进行富集性分析。结果各物种已知的成熟miR-16序列具有高度保守性。397个靶基因在分子功能上显著富集于蛋白结合、DNA结合、酶类活性、胰岛素样生长因子受体活性等功能,在生物学过程显著富集于发育过...

Objective The aim is to predict the target genes of has-miR-16 and to analyze the molecular function,biological process and signal pathway enrichment of target genes,and comprehensively know living activity process and disease associated with miR-16. Methods The collection of target genes were predicted with bioinformatics methods and relevant literature. Cytoscape 3.2. 1 and the database for annnotation visualization and integrated discovery(DAVID) database were used to analyze the GO(gene ontology) annotation and KEGG(kyoto encyclopedia of genes and genomes) signal pathway enrichment respect...