期刊论文

中文

剧本

0578-0659

2015

19

5

458-461

10.16462/j.cnki.zhjbkz.2015.05.008

国家自然科学基金青年基金资助项目（31200573）； 湖南省自然科学基金资助项目（13JJ6051）；

本校为第一机构

目的检测并观察蛋白质精氨酸甲基转移酶2(protein arginine methyltransferase 2,PRMT2)及其新的剪接体PRMT2α、PRMT2β、PRMT2γ在雌激素受体α(estrogen receptor α,ERα)阳性和ERα阴性乳腺癌细胞株中的表达情况。方法提取ERα阳性乳腺癌细胞株(ZR-75-l、BT474、T47D、MCF-7)和ERα阴性的乳腺癌细胞株(MDA-MB-453、SK-BR-3、MDA-MB-231)总RNA并用RT-PCR方法检测PRMT2及其新剪接体mRNA的表达,采用内对照甘油醛-3-磷酸脱氢酶(glyceraldehyde-3-phosphate dehydrogenase,GAPDH)同时进行相对定量以进行校正。结果不同乳腺癌细胞株中均可检测到PRMT2及其新剪接体的表达,在ERα阳性乳腺癌细胞株(...

Objective To investigate the expression of protein arginine methyhransferase 2 （PRMT2） and its novel variants PRMT2α,PRMT213,PRMT25, in different human breast cancer cell lines. Methods The total RNA was extracted from the ERα positive breast cancer cells （ZR-75-1, BT474,T47D, MCF-7） and ERct negative breast cancer cells （MDA-MB-453, SK-BR-3, MDA-MB-231 ） ,and real-time PCR was used to detect the expression of PRMT2 and its variants mRNA in these cells. Results The expressions of PRMT2 and its variants were detectable in different human...