期刊论文

Inhibitory effect of Qisheng-Yiqi Dropping Pill on apoptosis of myocardial cells in rats with chronic heart failure and its mechanism

Chen, J.

中文

吉林大学学报(医学版)

1671-587X

2020

46

5

972-978

10.13481/j.1671-587x.20200512

海南省科技厅重点研发计划项目资助课题（ZDYF2019121）；

本校为通讯机构

目的:探讨芪参益气滴丸(QSYQ)对慢性心力衰竭(CHF)大鼠心肌细胞凋亡的影响,并阐明其可能的作用机制。方法:60只雄性SD大鼠随机分为假手术组、模型组、阳性药对照组(6.75 mg·kg~(-1)·d~(-1)卡托普利)、低剂量(135 mg·kg~(-1)·d~(-1)) QSYQ组和高剂量(270 mg·kg~(-1)·d~(-1)) QSYQ组,每组12只。采用冠状动脉前降支结扎法建立CHF模型,造模成功后连续灌胃给药4周,超声心动图检测大鼠心功能,采用脱氧核糖核苷酸末端转移酶介导的缺口末端标志法(TUNEL)检测大鼠心肌细胞凋亡率,比色法测定大鼠血清中乳酸脱氢酶(LDH)和超氧化物歧化酶(SOD)活性及丙二醛(MDA)水平,流式细胞术检测大鼠心肌组织中活性...

Objective:To investigate the effect of Qishen-Yiqi Dropping Pill(QSYQ)on the apoptosis of myocardial cells in the rats with chronic heart failure(CHF),and to explore its possible mechanism. Methods:Sixty male SD rats were randomly divided into sham operationgroup,modelgroup,positivedrugcontrolgroup(6. 75 mg·kg-1 ·d-1 Captopril), low dose(135 mg·kg-1·d-1)of QSYQ group and high dose(270 mg·kg-1·d-1) of QSYQ group ,and there were 12 rats in each group. The CHF models were established by ligating anterior descending coronary artery. After suc...