期刊论文

中文

中医杂志

1001-1668

2022

63

2

164-168

10.13288/j.11-2166/r.2022.02.012

国家自然科学基金（81973917）； 湖南省自然科学基金（2020JJ5508）； 湖南省卫健委2020年度科研立项课题（20200474）；

本校为第一机构

目的探讨电针“百会”“人中”穴治疗脑缺血再灌注损伤(CIRI)的疗效及可能作用机制。方法36只雄性SD大鼠随机分为假手术组、模型组和电针组,每组12只。除假手术组外其余大鼠采用大脑中动脉线栓阻断法制备CIRI大鼠模型。造模24 h后对电针组大鼠行电针干预,穴取“百会”“人中”,频率15 Hz、强度为1 mA的连续波,每天1次,每次20 min,共5天。用Longa法评定各组大鼠神经功能损伤程度;TTC染色法测定大鼠脑梗死体积,HE染色观察脑组织病理形态改变;ELISA法检测脑脊液白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、白细胞介素18(IL-18)水平;逆转录聚合酶链反应和Western blotting法分别检测皮质缺血区脑组织...

Objective To explore the effect of electroacupuncture(EA)at Baihui(DU 20)and Renzhong(DU 26)on cerebral ischemia reperfusion injury(CIRI)and the possible mechanism. Methods A total of 36 male SD rats were randomized into sham-operation group(n=12),model group(n=12)and EA group(n=12). Suture middle cerebral artery occlusion was used to develop the CIRI model in all groups but the sham-operation group. Twenty-four hours after modeling,the rats in the EA group were performed for EA at Renzhong and Baihui,acupoints with a continuous wave with inten...