期刊论文

中文

癌变·畸变·突变

1004-616X

2019

31

6

483-487

10.3969/j.issn.1004-616x.2019.06.013

深圳市科技研发学科布局项目(JCYJ20170413101713324) 山西省重点研发计划项目(201803D31025)。

本校为第一机构

目的:探讨深圳和太原大气细颗粒物(PM2.5)样品是否具有致突变作用.方法:应用组氨酸缺陷型鼠伤寒沙门氏菌TA97、TA98、TA100、TA102和TA1535,通过平板渗入法同时对广东深圳和山西太原两地的PM2.5样品进行标准Ames试验.实验设4个PM2.5剂量组(分别为每皿40、100、200和400μg)、1个阴性对照组和1个阳性对照组,每种细菌均设立加S9和不加S9两种试验组.结果:深圳PM2.5样品的Ames试验结果显示,TA97、TA100和TA102在PM2.5样品处理后菌落回变数显著高于阴性对照组,加S9与不加S9的菌落回变数比较差异有统计学意义(P

OBJECTIVE:The Ames tests were used to investigate the mutagenicity of PM2.5 samples which were collected from Shenzhen and Taiyuan.METHODS:The histidine-deficient Salmonella typhimurium strains TA97,TA98,TA100,TA102 and TA1535 were used.From the collected samples,four doses of PM2.5(40,100,200,and 400μg per dish),a negative control and a positive control were tested.Each test group was treated with S9 or without S9.RESULTS:After treatment with PM2.5 samples from Shenzhen,there were significant differences in mutagenicity(P