期刊论文

中文

癌变·畸变·突变

1004-616X

2017

29

6

445-449

10.3969/j.issn.1004-616x.2017.06.008

深圳市科技研发基础研究项目

本校为第一机构

目的:研究邻苯二甲酸二(2-乙基己)酯(DEHP)对MCF-7细胞中3β-羟类固醇脱氢酶(3β-HSD)表达的影响。方法:采用不同浓度(0.05、0.1、0.2、0.4、0.8 mmol/L)DEHP对体外培养的MCF-7细胞分别染毒12、24和48 h,或同一浓度(0.8 mmol/L)染毒不同时间(3、6、12、24、48 h)后,荧光定量PCR检测各组MCF-7细胞3β-HSD mRNA表达水平,Western blot检测3β-HSD蛋白表达水平。结果:DEHP 0.1~0.8 mmol/L分别染毒细胞12~48 h,3β-HSD mRNA表达水平均较对照组升高,差异有统计学意义(P

OBJECTIVE: To study the effects of di- (2-ethylhexyl) phthalate (DEHP) on expression of 3β-hydroxysteroid dehydrogenase (3β-HSD) in MCF-7 cells. METHODS:MCF-7 cells in cultured were exposed to DEHP in different doses (0.05,0.1,0.2,0.4,0.8 mmol/L) for 12,24,48 h,or to one dose (0.8 mmol/L) at different times (3,6,12,24,48 h). Expression of 3β-HSD mRNA was detected by real-time PCR,expression of 3β-HSD protein was detected by western blot. RESULTS:After treatment of MCF-7 cells with DEHP at 0.1- 0.8 mmol/L for 12-48 h, expression levels of 3β...