期刊论文

中文

癌变·畸变·突变

1004-616X

2009

21

6

455-459

10.3969/j.issn.1004-616X.2009.06.012

本校为其他机构

背景与目的:观察苯和甲醛对胚胎发育的联合毒性作用,并探讨联合作用类型.材料与方法:采用体外全胚胎培养模型,按照3× 4析因设计的要求共分12组.将8.5日龄昆明种小鼠胚胎与含不同浓度苯和甲醛的即刻离心血清共培养48 h,观察苯和甲醛对小鼠胚胎生长发育和组织器官形态分化的影响及其对胚胎卵黄囊结构的损伤作用,并通过直观分析和方差分析判断苯与甲醛联合作用的类型.结果:空白和溶剂对照组培养胚胎与体内同龄胚胎的发育基本一致(P>0.05);苯和甲醛对小鼠胚胎发育均具有抑制作用,生长发育和器官分化各指标均低于对照组(P

BACKGROUND AND AIM： To explore the combined toxic effects of benzene and formaldehyde on mouse embryos in vitro. MATERIALS AND METHODS： Whole embryo culture system was adopted. According to 3 x 4 factorial design, 12 groups were set up. The 8.5-day isolated mouse embryos were cocultured for 48 hours in freshly centrifuged serum with different concentrations of benzene and formaldehyde. The growth, development and morphodifferentiation of tissues and organs of mouse embryos were studied. The type of combined toxic effects of benzene and formal...