期刊论文

中文

癌变·畸变·突变

1004-616X

1999

11

5

213-216

美国中华医学会（ C M B） 及国家自然科学基金；

本校为第一机构

本文介绍一种端粒酶检测方法，该方法是将 Ｔ Ｒ Ａ Ｐ法在 Ｐ Ｃ Ｒ 过程中引入３ Ｈｄ Ｃ Ｔ Ｐ，结合液闪计数ｃｐｍ 半定量分析端粒酶活性。应用该方法检测端粒酶阳性的 Ｃ Ｎ Ｅ２ 细胞和部分组织标本及 Ｒ Ｎａｓｅ Ａ 或加热预处理后的对照标本，并与 Ｔ Ｒ Ａ Ｐ 法相比较，结果显示 Ｃ Ｎ Ｅ２ 细胞端粒酶阳性，１０ 个 Ｃ Ｎ Ｅ２ 细胞中仍可检到端粒酶，组织样品的端粒酶与文献值基本一致；放射性活性计数（ｃｐｍ） 与 Ｃ Ｎ Ｅ２ 细胞抽提液中端粒酶活性具有良好的线性关系；用 Ｒ Ｎａｓｅ Ａ 或加热处理后标本为阴性，ｃｐｍ 值接近阴性对照；批内和批间变异度分别为１１ ．６...

This study developed a semi quantitative method of telomerase activity. The method was based on the combination of TRAP assay and scintillation count assay(\+3 H dCTP incorporation). It was used to quantify telomerase activity in CNE\-2 cells and some tissue specimens. RNase pretreated or heat treated cells were used as controls. The results demonstrated that telomerase activity measured by this method was positive in CNE\-2 cells, and it could be clearly detected with as few as 10 cells. There was a linear correlation between the radioactive count and the telomerase activity. The telomerase a...