期刊论文

Mechanism underlying the anti - apoptotic activity of pORF5 plasmid protein in Chlamydia trachomatis via high-mobility group box 1 protein: a preliminary study

中文

中华皮肤科杂志

0412-4030

2019

52

8

548-553

10.3760/cma.j.issn.0412-4030.2019.08.007

(81772210,31470277):国家自然科学基金 (201710555011):国家级大学生创新创业训练计划项目 (湘教通[2017]205号):湖南省大学生研究性学习和创新性实验计划项目 (81772210,31470277):National Natural Science Foundation of China (201710555011):National College Students' Innovation Entrepreneurship Training Program (Grant [2017]205):Hunan Provincial College Students Research Study and Innovative Experiment Program

本校为第一机构

目的 探讨pORF5质粒蛋白抗凋亡分子机制,为进一步阐明沙眼衣原体致病机制提供实验依据。方法 将HeLa细胞分为两组,一组用凋亡诱导剂碳酰氰基-对-氯苯腙（CCCP）刺激30 min,另一组经pORF5质粒蛋白预处理18 h后,再用CCCP刺激30 min。然后,Western印迹检测细胞凋亡相关蛋白Bcl-2、Bax和胱天蛋白酶3（caspase-3）的表达水平；JC-1荧光探针检测HeLa细胞线粒体膜电位变化；间接免疫荧光观察细胞色素c释放情况。为了分析高迁移率族蛋白1（HMGB1）是否参与pORF5质粒蛋白的抗凋亡作用,分别用HMGB1 shRNA和对照RNA稳定转染HeLa细胞,再用pORF5质粒蛋白与CCCP共刺激两种细胞后,测定Bcl-2、Bax和活化的caspas...

Objective To explore the molecular mechanism underlying the anti-apoptotic activity of pORF5 plasmid protein of Chlamydia trachomatis, so as to provide an experimental basis for further clarifying the pathogenesis of Chlamydia trachomatis. Methods HeLa cells were divided into two groups: carbonyl cyanide m-chlorophenyl hydrazone (CCCP, an apoptosis inducer) group was stimulated by CCCP for 30 minutes, and pORF5 + CCCP group was pre treated with pORF5 plasmid protein for 18 hours followed by CCCP for 30 minutes. Then, Western blot analysis was p...