期刊论文

中文

中华皮肤科杂志

0412-4030

2011

44

10

688-692

10.3760/cma.j.issn.0412-4030.2011.10.002

国家自然科学基金（30800996） 湖南省科技厅重点课题（2010FJ2008） 湖南省卫生厅课题（B2010-041）

本校为第一机构

目的探讨白介素2（IL-2）基因与梅毒螺旋体(Treponema pal_lidum，Tp)Gpd抗原编码基因融合构建双价核酸疫苗免疫新西兰兔后的免疫应答效果及感染Tp后的保护作用。方法定向克隆构建真核表达重组体pcDNA3.1(+)/Gpd-IL-2，与先期构建的pcDNA3.1(+)/Gpd真核表达重组体分别设为两个疫苗实验组，同时设pcDNA3.1(+)空质粒对照组及PBS对照组，共4组，每组18只雄性新西兰兔，肌肉多点注射初次免疫，于初次免疫后第10周各实验组兔皮下接种Tp标准株进行感染实验，酶联免疫吸附试验(ELISA)检测不同时间点免疫兔特异性抗体产生水平和脾细胞IL-2及干扰素γ(IFN-γ)诱导水平，噻唑蓝法检测兔脾淋巴细胞增殖水平...

Objective To investigate the immune response to and protective effect of a bivalent DNA vaccine expressing interleukin-2(IL-2)and Gpd proteins in New Zealand rabbits.Methods Seventy-two male New Zealand white rabbits were equally and randomly divided into 4 groups to be immunized with recombinant plasmids pcDNA3.1(+)/Gpd-IL-2(pcD/Gpd-IL-2),pcDNA3.1(+)/Gpd(pcD/Gpd),empty plasmid pcDNA3.1(+)(pcD)and phosphate buffered saline(PBS),respectively.Immunization was carried out by intramuscular injection at multiple sites with a 2-week interval for 3 ti...