期刊论文

中文

中华皮肤科杂志

0412-4030

2004

37

12

692-694

10.3760/j.issn:0412-4030.2004.12.003

湖南省教育厅资助重点课题（02A046）； 湖南省卫生厅资助课题（Y02-067）；

本校为第一机构

目的了解湖南衡阳和广东江门地区梅毒螺旋体(TP)基因亚型的分布.方法收集衡阳和江门地区疑似硬下疳病例的标本85份,经TP polA PCR筛选后,阳性者用PCR扩增各标本中TP的arp和tpr基因,酶切tpr基因扩增产物,分析各TP菌株arp基因长度和tpr基因的限制性片段长度多态性(RFLP),进行分型.结果TP polA PCR阳性者69例,可用于分型者57例,分为10个亚型,其中14d亚型为26例,其他亚型包括10d(1),12a(3),12g(2),13d(6),14a(5),14b(2),14f(6),15d(5),16d(1);衡阳和江门地区TP基因亚型分布大致相同.结论衡阳和江门地区存在TP的多亚型,都以14d亚型为主(占45.6%),无明显地区性差异.

Objective To investigate the distribution of subtypes of T. pallidum (TP) in Hengyang and Jiangmen regions. Methods Eighty-five specime ns taken from patients with suspected chancre collected in Hengyang and Jiangmen from February 2002 to January 2004 were screened by a PCR targeted TP polA gene , and then the arp gene and tpr gene were amplified from TP positive specimens. The PCR products of tpr gene were digested by restriction endonuclease MSe I. Th e sizes of the arp gene and the restriction fragment length polymorphism (RFLP) of the tpr gene were analyzed for subtyping. Results Of 69 TP-...