期刊论文、会议论文

中文

中华实验和临床病毒学杂志

1003-9279

2008

22

4

275-277

全国第2届中西医结合传染病学术会议暨国家中医药管理局第1届传染病协作组会议

全国第2届中西医结合传染病学术会议暨国家中医药管理局第1届传染病协作组会议论文集

2008-11-01

北京

中国中西医结合学会

10.3760/cma.j.issn.1003-9279.2008.04.012

国家教育部留学回国人员基金（编号2006KY12.1.4.2）；

本校为其他机构

目的 研究乙型肝炎病毒C蛋白在NK细胞中的表达及其对NK细胞功能的影响.方法 用脂质体转染法将HBV C基因真核表达载体pHBI-CMV-HBC转入NK-92细胞,通过WesternBlot检测C基因在NK-92细胞中表达,用ELISA法检测NK细胞分泌IFN-γ水平,MTT法检测NK细胞对HepG2细胞的细胞毒作用.结果 Western Blot证实转染有pHBI-CMV-HBC的NK-92细胞能表达HBV C蛋白.转染了重组真核表达质粒的NK-92细胞IFN-γ水平均高于空质粒对照组和空白对照组(P

OBJECTIVE: To investigate the influence of hepatitis B virus C protein on the function of natural killer cell. METHODS: Recombinant eukaryotic expression plasmid pHBI-CMV-HBC was constructed and confirmed by double restrictive enzyme digestion and DNA sequencing analysis. Then the recombinant plasmid was transfected into NK-92 cells with lipofectamine encapsuled. The transfected NK-92 cells containing expressive HBV C protein was confirmed by Western Blot analysis. ELISA was employed to determine the IFN-gamma level secreted by NK-92 cells. And finally the cytotoxicities of NK cells were analy...