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Three polypeptides screened from phage display random peptide library may be the receptor polypeptide of Mycoplasma genitalium adhesion protein

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成果类型:
期刊论文
作者:
Deng, Xiangying;Zhu, Youcong;Dai, Pei;Yu, Minjun;Chen, Liesong;...
通讯作者:
Zeng, Yanhua
作者机构:
[Dai, Pei; Zhu, Youcong; Deng, Xiangying; Chen, Liesong; Zhu, Cuiming; Yu, Minjun; You, Xiaoxing; Zeng, Yanhua; Li, Lingling] Univ South China, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Inst Pathogen Biol,Med Coll, Hengyang 421001, Peoples R China.
通讯机构:
[Zeng, Yanhua] U
Univ South China, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Inst Pathogen Biol,Med Coll, Hengyang 421001, Peoples R China.
语种:
英文
关键词:
adhesin;aspartyltryptophylserylseryltryptophylvalyltyrosylarginylaspartylprolylglutamylthreonine polypeptide;histidyltyrosylisoleucylaspartylphenylalanylarginyltryptophan polypeptide;polypeptide;protein antibody;recombinant protein;unclassified drug;valylhistidyltryptophylaspartylphenylalanylarginylglutamyltryptophyltrytophylglutamylprolylserine polypeptide;adhesin;bacterial protein;peptide;protein binding;Article;cell adhesion;cell interaction;controlled study;enzyme linked immunosorbent assay;host cell;human;human cell;immunofluorescence microscopy;in vitro study;Mycoplasma genitalium;nonhuman;peptide library;phage display;priority journal;protein binding;protein function;receptor binding;SV-HUC-1 cell line;antibody specificity;bacteriophage;bacterium adherence;cell line;chemistry;metabolism;Mycoplasma genitalium;mycoplasmosis;Adhesins, Bacterial;Antibody Specificity;Bacterial Adhesion;Bacterial Proteins;Bacteriophages;Cell Line;Enzyme-Linked Immunosorbent Assay;Humans;Mycoplasma genitalium;Mycoplasma Infections;Peptide Library;Peptides;Protein Binding;Recombinant Proteins
期刊:
Microbial Pathogenesis
ISSN:
0882-4010
年:
2018
卷:
120
页码:
140-146
基金类别:
This work was supported by the National Natural Science Foundation of China (no: 31370207 ).
机构署名:
本校为第一且通讯机构
院系归属:
医学院
药学与生物科学学院
摘要:
Mycoplasma genitalium adhesion protein (MgPa) is a major adhesin of M. genitalium, a human pathogen associated with a series of genitourinary tract diseases. MgPa plays a very important role in M. genitalium adhering to the host cells. However, the exact receptor peptides or proteins of MgPa are still poorly understood so far. Three polypeptides (V-H-W-D-F-R-Q-W-W-Q-P-S), (D-W-S-S-W-V -Y-R-D-P-Q-T) and (H-Y-I-D-F-R-W) were previously screened from a phage display random peptide library using recombinant MgPa (rMgPa) as a target molecule. In thi...

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