Rational design of heterodimeric protein using domain swapping for myoglobin

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成果类型：

期刊论文

作者：

Lin, Ying-Wu;Nagao, Satoshi;Zhang, Mohan;Shomura, Yasuhito;Higuchi, Yoshiki;...

通讯作者：

Hirota, Shun

作者机构：

[Hirota, Shun; Nagao, Satoshi; Lin, Ying-Wu; Zhang, Mohan] Nara Inst Sci & Technol, Grad Sch Mat Sci, Ikoma, Nara 6300192, Japan.

[Lin, Ying-Wu] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.

[Higuchi, Yoshiki; Shomura, Yasuhito] Univ Hyogo, Grad Sch Life Sci, Dept Life Sci, Kakogawa, Hyogo 6781297, Japan.

[Higuchi, Yoshiki; Shomura, Yasuhito] RIKEN SPring 8 Ctr, Sayo, Hyogo 6795148, Japan.

[Hirota, Shun] Nara Inst Sci & Technol, Grad Sch Mat Sci, 8916-5 Takayama, Ikoma, Nara 6300192, Japan.

通讯机构：

[Hirota, Shun] N

Nara Inst Sci & Technol, Grad Sch Mat Sci, 8916-5 Takayama, Ikoma, Nara 6300192, Japan.

语种：

英文

关键词：

dimerization;heme coordination;heme proteins;salt bridges;X-ray crystallography

期刊：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

ISSN：

1433-7851

年：

2015

卷：

期：

页码：

511-515

DOI：

10.1002/anie.201409267

基金类别：

Japan Society for the Promotion of ScienceMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of Science [26288080, 24750163]; JSPS (Invitation Fellowship Program); Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370812]; Grants-in-Aid for Scientific ResearchMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [26288080, 24750163] Funding Source: KAKEN

机构署名：

本校为其他机构

院系归属：

化学化工学院

摘要：

Protein design is a useful method to create novel artificial proteins. A rational approach to design a heterodimeric protein using domain swapping for horse myoglobin (Mb) was developed. As confirmed by X-ray crystallographic analysis, a heterodimeric Mb with two different active sites was produced efficiently from two surface mutants of Mb, in which the charges of two amino acids involved in the dimer salt bridges were reversed in each mutant individually, with the active site of one mutant modified. This study shows that the method of constru...

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Rational design of heterodimeric protein using domain swapping for myoglobin

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