期刊论文、会议论文

Huang, Bo

中文

中国烟草学报

1004-5708

2015

21

2

100-104

中国毒理学会第四届中青年学者科技论坛

2014-8-13

银川

中国毒理学会

10.3969/j.issn.1004-5708.2014.160

国家自然科学基金资助(No.81272994)

本校为第一且通讯机构

目的:研究卷烟烟气所致人支气管上皮细胞DNA损伤以及茶多酚二甲基亚砜合剂的保护作用,为卷烟烟气对机体损伤的防护提供理论及实验依据。方法将细胞分为5组:空白组、卷烟烟气组、茶多酚保护组、二甲基亚砜保护组、茶多酚二甲基亚砜合剂保护组;通过检测微核和多核细胞观察染色体损伤,彗星实验及H2AX蛋白磷酸化检测DNA的损伤,多核细胞法检测细胞HPRT突变。结果BEAS-2B细胞经卷烟烟气染毒后,细胞的微核率、多核率、拖尾率升高,H2AX蛋白磷酸化表达增高, HPRT突变率增高,差异均有统计学意义(P

Cigarette smoke condensates(CSC)induced DNA damage in BEAS-2B cells and protective effect of tea polyphenols DMSO mixture were studied with an objective to offer theoretical and experimental basis for protection against CSC-induced damage. BEAS-2B cells were divided into 5 groups, i.e. control, CSC, tea polyphenol protection, DMSO protection and TPs DMSO mixture protection. Chromosomal damage was determined by measuring the frequency of micronuclei and multinucleated cells. DNA damage was examined by comet assay and level of γH2AX. Gene mutati...