期刊论文

The effect of PM2.5 on apoptosis gene expression in HBE cells

中文

职业与健康

1004-1257

2019

35

17

2332-2336

10.13329/j.cnki.zyyjk.2019.0621

深圳市科技研发学科布局项目(JCYJ20170413101713324).

本校为第一机构

目的 探讨PM2.5对支气管上皮(HBE)细胞凋亡基因表达的影响.方法 用PM2.5低剂量10 μg/mL和高剂量50 μg/mL对HBE细胞染毒,用未染毒的细胞作为对照组,10 μg/mL的Cr6+为阳性对照组,应用实时荧光定量聚合酶链式反应(qRTPCR)检测凋亡基因Caspase-3、Caspase-8、Caspase-9、Bax、Bcl-2的mRNA表达水平变化情况,Western blot检测凋亡基因的蛋白质表达变化情况.结果 qRT-PCR结果显示,与对照组比较,PM2.5 10μg/mL和50 μg/mL染毒以及阳性对照组染毒后,Caspase-3基因表达量分别升高193.0％、340.0％、214.0％,Caspase-8基因表达分别升高50.0％、84.0％、71.0％,Caspase-9基因表达分别升高94.0％、177.0％...

Objective To understand the effect of particulate matter 2.5(PM2.5)on apoptosis gene expression in human bronchial epithelial(HBE)cells.Methods HBE cells were treated with PM2.5 for 24 h at 10μg/mL and 50μg/mL,additionally,cells were treated with blank as negative control,10μg/mL Cr6+as a positive control.After the treatment,m RNA expression of apoptosis genes including Caspase-3,Caspase-8,Caspase-9,Bax,and Bcl-2 were detected by fluorescent quantitative RT-PCR(qRTPCR),the protein expression of apoptosis genes were detected with Western blot...