期刊论文

Effects of oxidative damage in RAW264.7 macrophage cells induced by uranium dusts

中文

职业与健康

1004-1257

2013

29

1

1-4

10.13329/j.cnki.zyyjk.2013.01.016

国家自然科学基金资助项目（项目编号:30670634）；

本校为第一机构

目的 探讨铀矿尘作用于小鼠RAW 264.7细胞一定时间后羟自由基(·OH)、过氧化氢(H2O2)及丙二醛(MDA)含量的变化.方法 用120 μg/ml的铀矿尘混悬液作用于小鼠RAW264.7细胞,分别在作用2、4、8、16、24和32 h后,采用噻唑蓝比色法(MTT法)检测RAW264.7细胞存活率；HE染色镜下观察细胞形态学变化；比色法测定细胞上清液中·OH、H2O2及MDA含量的改变.结果 铀矿尘作用于RAW264.7细胞2、4、8、16、24、32 h后,可见铀矿尘对RAW264.7细胞的增殖有明显抑制作用,染尘32 h后细胞存活率仅有(28.37±3.3)％；铀矿尘在作用于RAW264.7细胞16、24、32 h后,镜下可见梭型或不规则型细胞、多核细胞明显增加,细胞胞质缺失...

[Objective]To explore the changes of·OH,H2O2 and MDA levels in RAW264.7 murine macrophage cells after exposure to uranium dusts for a certain period of time.[Methods]RAW264.7 murine macrophage cells were treated with uranium dust in aqueous suspension 120 μg/ml.After treatment for 2,4,8,16,24 and 32 h,the rate of survival RAW264.7 was measured with MTT method.Morphological changes were observed by microscope in HE staining.The levels of ·OH、H2O2 and MDA in extracellular were detected by colorimetric method.[Results] Treated with 120 μg/ml ...