期刊论文

Cao, R.X.

中文

CANCER COMMUNICATIONS

1000-467X

2008

27

8

822-827

10.3321/j.issn:1000-467X.2008.08.007

湖南省自然科学基金资助项目（No.06jj5046）~~；

本校为第一机构

背景与目的：有研究表明，S100A13基因与肿瘤的发生有关，而S100A13基因在人甲状腺组织中高表达。本研究旨在探讨S100A13基因过表达对甲状腺癌TT细胞增殖特性的影响。方法：应用Lipofectamine 2000将真核表达载体pCDNA3．1／NT—GFP—S100A13和空载体pCDNA3．1／NT-GFP导入TT细胞，经G418抗性筛选得到稳定的克隆并扩大培养成细胞系，激光共聚焦显微镜观察外源性S100A13蛋白在细胞中的定位。采用real—time RT-PCR和Western blot鉴定稳定过表达S100A13的TT细胞。分别应用细胞生长曲线、流式细胞术方法检测过表达S100A13基因对TT细胞生长速率和细胞周期的影响。结果：成功建立了稳定过表达S100A13...

BACKGROUND & OBJECTIVE: S100A13 is involved in tumor formation, and is highly expressed in human thyroid gland. This study was to investigate the effect of exogenous S100A13 overexpression on the proliferation of human thyroid cancer cell line TT. METHODS: The eukaryotic expression plasmid pCDNA3.1/NT-GFP-S100A13 and empty vector pCDNA3.1/NT-GFP were transfected into TT cells. The cells were selected by G418. The expression of green fluorescent protein (GFP) was observed under laser scanning microscope, and the expression of S100A13 mRNA and protein was detected by real-time reverse transcript...