This paper simulated and analyzed the laboratory animal room by CFD software, studies the best air change rate in laboratory room, and analyzed the energy-conservation potential of the air conditioning for laboratory animal room. The results indicated the best air changes of laboratory animal room is 14 times/h which take the air distribution of supplying in ceiling diffuser and exhausting air in underside, compare air change rates of 20 times/h which taken in old system, 33.43...