Construction of Biocatalysts Using the P450 Scaffold for the Synthesis of Indigo from Indole

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成果类型：

期刊论文

作者：

Li, Yanqing;Lin, Yingwu;Wang, Fang;Wang, Jinghan;Shoji, Osami;...

通讯作者：

Jiakun Xu<&wdkj&>Osami Shoji

作者机构：

[Li, Yanqing] Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China.

[Li, Yanqing; Wang, Fang; Wang, Jinghan; Xu, Jiakun] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fisheries, Lab Marine Drugs & Byprod,Minist Agr & Rural Affai, Qingdao 266071, Peoples R China.

[Lin, Yingwu] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.

[Wang, Jinghan] Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China.

[Shoji, Osami] Nagoya Univ, Grad Sch Sci, Dept Chem, Furo Cho,Chikusa Ku, Nagoya 4648602, Japan.

通讯机构：

[Jiakun Xu; Osami Shoji] A

Authors to whom correspondence should be addressed.<&wdkj&>Department of Chemistry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Key Lab of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Lab for Marine Drugs and Byproducts of Pilot National Lab for Marine Science and Technology, Qingdao 266071, China

语种：

英文

关键词：

cytochromes P450BM3;dual-functional small molecule co-catalysis;hydroxylated indole;indigo;directed evolution

期刊：

International Journal of Molecular Sciences

ISSN：

1422-0067

年：

2023

卷：

期：

页码：

2395-

DOI：

10.3390/ijms24032395

基金类别：

Data curation, Y.L. (Yanqing Li) and J.W.; Formal analysis, Y.L. (Yanqing Li) and F.W.; Funding acquisition, J.X.; Methodology, Y.L. (Yanqing Li) and J.X.; Writing—original draft, Y.L. (Yanqing Li); Writing—review and editing, Y.L. (Yingwu Lin), O.S. and J.X. All authors have read and agreed to the published version of the manuscript. This research was funded by the National Natural Science Foundation of China (No. 32171263) and the Central Public-interest Scientific Institution Basal Research Fund, CAFS (No. 2020TD67).

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本校为其他机构

院系归属：

化学化工学院

摘要：

With the increasing demand for blue dyes, it is of vital importance to develop a green and efficient biocatalyst to produce indigo. This study constructed a hydrogen peroxide-dependent catalytic system for the direct conversion of indole to indigo using P450BM3 with the assistance of dual-functional small molecules (DFSM). The arrangements of amino acids at 78, 87, and 268 positions influenced the catalytic activity. F87G/T268V mutant gave the highest catalytic activity with k(cat) of 1402 min(-1) and with a yield of 73%. F87A/T268V mutant was found to produce the indigo product with chemosele...

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Construction of Biocatalysts Using the P450 Scaffold for the Synthesis of Indigo from Indole

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