期刊论文

Differentially expressed circular RNAs in human gastric cancer cells

中文

生理学报

0371-0874

2018

70

4

384-390

10.13294/j.aps.2018.0041

supported by the Second Batch of Research Projects in 2017 from Hunan Provincial Health and Family Planning Commission（No.A2017014） the National Natural Science Foundation of China（No.81071965）

本校为第一机构

本文旨在探讨环状RNA (circular RNA, circ RNA) 在不同分化程度的胃癌细胞MGC-803、SGC-7901、NCI-N87与正常胃上皮细胞GES-1中的表达谱变化.体外培养低分化胃癌细胞MGC-803、中分化胃癌细胞SGC-7901、高分化胃癌细胞NCI-N87和正常胃上皮细胞GES-1, 利用高通量circ RNA芯片技术检测三种不同分化程度胃癌细胞和正常胃上皮细胞中差异表达的circ RNA, 借助生物信息学软件预测可能与circ RNA相互作用的微小RNA (micro RNA, mi RNA), 结合文献检索初步选定可能在胃癌中具有重要意义的circ RNA.结果显示, 与正常胃上皮细胞相比, 在不同分化程度胃癌细胞中均表达上调的circ RNA有79条, 均表达下调的有...

The purpose of this study was to investigate the expression profile of circular RNA (circRNA) in gastric cancer cells MGC-803, SGC-7901 and NCI-N87 with different degrees of differentiation and normal gastric epithelial cells GES-1. High throughput circRNA microarray technique was used to detect the differential expression of circRNA between three kinds of differentiated gastric cancer cells and normal gastric epithelial cells. The interaction of microRNAs (miRNAs) with circRNAs was predicted by bioinformatics software, and circRNA, which might have great significance in gastric cancer, was id...