期刊论文

中文

生理学报

0371-0874

2015

67

2

193-200

10.13294/j.aps.2015.0023

(No.81173060):This work was supported by the National Natural Science Foundation of China (No.2014-405):and Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study,China

本校为第一机构

血管紧张素II (angiotensin II, Ang II)和降钙素基因相关肽(calcitonin gene-related peptide, CGRP)在血管的损伤和保护中起重要作用。为了探讨CGRP受体成分蛋白(receptor component protein, RCP)在CGRP和Ang II对血管平滑肌细胞(vascular smooth muscle cell, VSMC)血管过氧化物酶1 (vascular peroxidase-1, VPO1)表达调控中的作用及机制,本研究采用体外培养鼠源性A10血管平滑肌细胞株(A10VSMC),给予CGRP或/和Ang II刺激,并用小干扰RNA (siRNA)干扰细胞RCP基因的表达, Western Blot检测RCP以及VPO1蛋白表达水平;RT-PCR检测RCP及VPO1 mRNA的表达水平。结果显示,在静止期野生型A10VSMC,CGRP和...

Angiotensin II (Ang II) and calcitonin gene-related peptide (CGRP) play important roles in vascular injury and protection. In order to determine the role of CGRP receptor component protein (RCP) in signal transduction whereby CGRP and Ang II mediate the expression of vascular peroxidase-1 (VPO1) in vascular smooth muscle cell (VSMC), mouse derived A10 vascular smooth muscle cell line (A10VSMC) was cultured with CGRP or/and Ang II in vitro. RCP-specific small interference RNA (siRNA-RCP) was used to silence oligonucleotide sequence. Western blot and RT-PCR were used to determine the protein and...