期刊论文

Qin, L.

中文

生理学报

0371-0874

2006

58

1

47-52

10.3321/j.issn:0371-0874.2006.01.008

This work was supported by the National Natural Science Foundation of China （No. 30470719）.；

本校为第一机构

本文旨在观察普伐他汀对鼠源巨噬细胞性泡沫细胞内胆固醇酯含量的影响，探讨此作用与小凹蛋白一l的关系。采用体外培养的鼠源性巨噬细胞株作为研究对象，加入氧化低密度脂蛋白（oxidized low density lipoprotein，OX-LDL）使其形成泡沫细胞，运用高效液相色谱测定细胞内胆固醇酯的改变，同时运用Western blot检测细胞中小凹蛋白-1的表达，并观察普伐他汀对细胞内胆固醇酯和小凹蛋白-1影响的量效和时效关系。结果显示：普伐他汀可明显降低泡沫细胞内的胆固醇酯与总胆固醇的比值，且在一定范围内呈剂量依赖性和时间依赖性。在泡沫细胞中加入普伐他汀后能够促进小凹蛋白-1的表达，呈剂量依赖性和时...

The purpose of the present study was to investigate the effect of pravastatin on cholesteryl esters in foam cells of murine macrophages and the relation with caveolin-1. RAW 264.7 murine macrophages were coincubated with 80 mg/L oxidized low density lipoprotein (ox-LDL) and pravastatin (0~100 mumol/L) respectively for 24 h. When the best control concentration of pravastatin was confirmed, RAW 264.7 murine macrophages were coincubated with 80 mg/L ox-LDL and pravastatin of the best concentration respectively for 0, 6, 12, 24 h. Oil red O dyeing experiment was used to show the lipid droplets in ...