期刊论文

Lin, Ying-Wu

英文

ACS CATALYSIS

2155-5435

2020

10

1

891-896

10.1021/acscatal.9b05080

It is a pleasure to acknowledge Prof. S. G. Sligar and Prof. Y. Lu of University of Illinois at Urbana–Champaign, for the kind gift of the sperm whale Mb gene. X-ray diffraction data were collected at Shanghai Synchrotron Radiation Facility (SSRF), China. EPR studies were performed on the Steady High Magnetic Field Facilities, High Magnetic Field Laboratory, Chinese Academy of Science. This work was supported by the National Natural Science Foundation of China (21977042), Special Scientific Research Funds for Central Nonprofit Institutes, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (20603022016011), Financial Fund of the ministry of Agriculture and Rural Affairs, China (NFZX2018), and Human Provincial Innovation Foundation for Postgraduate (CX20190729).

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Functional enzyme design has made tremendous progress, but designer enzymes with activities comparable to those of natural enzymes are still limited. In this study, we rationally engineered a functional peroxidase with a catalytic binding site of guaiacol in a model protein, myoglobin (Mb), by replacing Phe46 with a serine (F46S mutation), together with a distal Tyr in the heme pocket (F43Y mutation). The double mutant F43Y/F46S Mb exhibited an overall catalytic efficiency that exceeds most natural peroxidases and is similar to the most efficient horseradish peroxidase. The catalytic substrate...