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The N-terminal D1 domain of Treponema pallidum flagellin binding to TLR5 is required but not sufficient in activation of TLR5.

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成果类型:
期刊论文
作者:
Xu, Man;Xie, Yafeng;Tan, Manyi;Zheng, Kang;Xiao, Yongjian;...
通讯作者:
Wu, Yimou
作者机构:
[Wu, Yimou; Zhao, Feijun; Xie, Yafeng; Zheng, Kang; Xu, Man; Zeng, Tiebing] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hunan Prov Key Lab Special Pathogens Prevent & Co, Inst Pathogen Biol,Med Coll, Hengyang, Peoples R China.
[Xie, Yafeng; Xiao, Yongjian] Univ South China, Affiliated Hosp 2, Dept Clin Lab, Hengyang, Peoples R China.
[Tan, Manyi] Hunan Prov Ctr Dis Control & Prevent, Changsha, Hunan, Peoples R China.
[Jiang, Chuanhao] Cent South Univ, Xiangya Hosp 2, Dept Lab Med, Changsha, Hunan, Peoples R China.
[Wu, Yimou] Univ South China, Med Coll, Inst Pathogen Biol, Hengyang 421001, Hunan, Peoples R China.
通讯机构:
[Wu, Yimou] U
Univ South China, Med Coll, Inst Pathogen Biol, Hengyang 421001, Hunan, Peoples R China.
语种:
英文
关键词:
amino acid;bacterial vaccine;binding protein;flagellin;interleukin 6;interleukin 8;plasmid DNA;recombinant protein;toll like receptor 5;flagellin;interleukin 6;interleukin 8;protein binding;TLR5 protein, human;toll like receptor 5;amino terminal sequence;Article;chromatin immunoprecipitation;comparative study;controlled study;gene;gene mutation;human;human cell;inflammation;mutagenesis;priority journal;protein binding;protein domain;real time reverse transcription polymerase chain reaction;signal transduction;syphilis;three dimensional imaging;TLR5 gene;transient expression;Treponema pallidum;Western blotting;amino acid sequence;cell line;genetic transcription;genetics;metabolism;syphilis;THP-1 cell line;Treponema pallidum;Amino Acid Sequence;Cell Line;Flagellin;Humans;Interleukin-6;Interleukin-8;Protein Binding;Signal Transduction;Syphilis;THP-1 Cells;Toll-Like Receptor 5;Transcription, Genetic;Treponema pallidum
期刊:
Journal of Cellular and Molecular Medicine
ISSN:
1582-1838
年:
2019
卷:
23
期:
11
页码:
7490-7504
基金类别:
This work was supported by the National Natural Science Foundation of China (Grant Nos. 81471576, 81702046 and 81373230), the Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study (Grant No. 2015- 351), the Natural Science Foundation of Hunan Province (Grant No. 2019JJ50535), the Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control Foundation (Grant No. 2014-5) and Graduate Student Research Innovation Project of Hunan Province (Grant No. CX2018B583).
机构署名:
本校为第一且通讯机构
院系归属:
医学院
药学与生物科学学院
摘要:
Syphilis is a chronic bacterial infection caused by Treponema pallidum (Tpallidum) and the pathogenesis that Tpallidum infection induces immunopathological damages in skin and other tissues remains unclear. We have previously reported that recombinant flagellins of Tpallidum can elicit IL-6 and IL-8 transcriptions via TLR5 pathway. To identify the domains which induced the pro-inflammatory activity and the importance of the interactions between TLR5 and domains, homology-based modelling and comparative structural analyses revealed that Tpflagel...

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