期刊论文

Study on content of major chemical constituents and anti - oxidation activities of Sea buckthorn fruit

中文

华西药学杂志

1006-0103

2020

35

5

513-517

10.13375/j.cnki.wcjps.2020.05.010

ZDSYS201707281432317:深圳市科技计划 CKFW2016082916015476:深圳市科技计划

本校为第一机构

目的 考察沙棘果中单一黄酮(槲皮素、山奈酚、异鼠李素)与总黄酮含量的一致性,及其抗氧化活性与主要成分含量的相关性.方法 采用HPLC法测定单一黄酮及维生素C的含量,检测波长分别为360、254 nm;按2015年版《中国药典》中的方法测定总黄酮的含量;以维生素E乙酸酯的抗铜离子(Cu2+)氧化能力测定不同样品的体外抗氧化活性.结果 10个不同产地沙棘果中单一黄酮、总黄酮、维生素C的含量及体外抗氧化活性均存在明显差异.结论 沙棘果中单一黄酮及总黄酮的含量不具备一致性,其抗氧化活性与总黄酮和维生素C的含量具有良好的相关性.

OBJECTIVE To investigate the consistency of total flavonoid with single flavonoid (quercetin,kaempferol and isorhamnetin) in Sea buckthorn fruit by comparing their contents,and to investigate the correlation between antioxidant activity and its main components.METHODS The single flavonoids and Vitamin C in Sea buckthorn fruit were determined by HPLC at 360 nm and 254 nm,respectively. The total flavonoids were measured according to Chinese Pharmacopoeia. Anti - oxidation activities of different samples were assayed by using Trolox method with the anti - copper ion (Cu~(2 +)) oxidation ability. ...