期刊论文

中文

环境与职业医学

2095-9982

2012

29

4

243-245

10.13213/j.cnki.jeom.2012.04.016

本校为第一机构

[目的]研究铅对人肾小管上皮细胞( human kidney cells,HK-2)线粒体的损伤作用,并探讨其可能的作用机制. [方法]以不同浓度的乙酸铅处理体外培养的HK-2细胞,罗丹明123( rhodamine 123,Rh123)及10-壬基吖啶橙(10-nonyl acridine orange,NAO)结合荧光化学发光仪分别检测细胞线粒体膜电位及心磷脂水平,细胞免疫荧光法结合流式细胞术检测线粒体细胞色素c( cytochrome c,Cytc)释放情况,加甘露醇观察铅对心磷脂及Cytc变化的影响.[结果]乙酸铅能造成HK-2细胞线粒体膜电位剂量及时间依赖性下降,荧光强度从正常对照组的4.56±0.25下降到400 μmol/L组的2.90士0.26或从正常对照组的4.44±0.20下降到12h组的...

[Objective] To explore the possible mechanism of mitochondria damage in human kidney cells（HK-2 cells） induced by lead acetate in vitro.[Methods] Cultured HK-2 cells were exposed to different level of lead acetate.Mitochondrial membrane potential（MMP） and diphosphatidyl glycerol were detected by rhodamine 123（Rh123） and 10-nonyl acridine orange（NAO）.The release of cytochrome c（Cyt c） from mitochondria was detected by flow cytometry combined with immunofluorescence.Changes of diphosphatidyl glycerol and Cyt c were observed with mannito...