期刊论文

Effects of death domain-associated protein on vascular stenosis and smooth muscle cells proliferation in rats after carotid angioplasty

中文

临床与病理杂志

2095-6959

2018

38

6

1146-1152

10.3978/j.issn.2095-6959.2018.06.002

湖南省自然科学基金（2011JJ2117）~~；

本校为第一机构

目的:探讨大鼠颈动脉成形术后,死亡域相关蛋白（death domain-associated protein,DAXX）对损伤后血管狭窄和平滑肌细胞增殖的影响及其机制。方法:将32只SD大鼠随机分为4组:假手术组、手术组、空转染组、转染组。所有动物暴露颈动脉,假手术组仅游离颈总动脉,手术组采用球囊扩张损伤大鼠左颈总动脉,空转染组及转染组在此基础上局部运用脂质体转染DAXX空质粒或DAXX质粒,于术后7,14 d分别取各组大鼠左颈总动脉行HE染色,观察并测量内膜、中膜的面积比;免疫组织化学和PCR检测平滑肌中DAXX及氨基末端激酶（Jun N-terminal kinase,JNK）的表达。结果:术后7,14 d手术组均出现血管管腔狭窄,管壁增厚,血管...

Objective： To investigated the effects and the mechanism of death domain-associated protein（DAXX） on vascular stenosis and smooth muscle cells proliferation in the balloon-injured rat carotid artery. Methods： Thirtytwo male SD rats were divided into 4 groups： a sham-surgery group, a surgery group, a blank-transfected group, and a transfected group. The gross artery in the sham-surgery group were free, in the surgery group the carotid artery were injured by using balloon, based on the above the blank-transfected group and the transfected gr...