期刊论文

Silencing RORαpromote proliferation, migration and invasion through Wnt signaling pathway in MGC803 cells

中文

临床与病理杂志

2095-6959

2016

36

10

1562-1568

10.3978/j.issn.2095-6959.2016.10.019

国家自然基金（81374013） 湖南省卫计委科研课题（B2015-182）.

本校为第一机构

目的：在构建沉默RORα人胃癌MGC803细胞的基础上，观察沉默RORα对MGC803细胞增殖与迁移侵袭的影响。方法：软琼脂集落形成实验、流式细胞术、细胞迁移和侵袭实验检测RORα沉默对MGC803细胞增殖、细胞周期、迁移和侵袭能力的影响。RT-PCR与Western blot检测RORα、Wnt1、MMP-9与TIMP3 mRNA与蛋白表达。结果：集落形成实验显示，沉默组的集落形成率128.1%明显高于对照组和空载体组(P

Objective:To investigate Silencing RORαthrough Wnt signaling pathway promote proliferation, migration and invasion in human gastric MGC803 cells underlaying construction of MGC803 cells of silencing RORα. Methods:Colony formation and lfow cytometry were used for cell proliferation and cell cycle. Wound healing and transwell assays were performed to examine cell migration and invasion activities. hTe expression of RORα, Wnt1, MMP-9 and TIMP3 mRNA and protein were detected by Western blot and RT-PCR. Results:hTe colony forming showed that the ...